MicroRNA Regulation of Oxidative Stress, Proliferation, and Neural Tube Closure

single-cell sequencing of the mouse cranial region at E8.25 (the start of neural tube closure, at E9.5 (the end of neural tube closure, and of a miR-302 knockout embryo at E9.5 (example of neural tube closure defrect). The cranial region anterior to the otic placode was dissected for wildtype embryos at E8.25 and E9.5 and for a miR-302 knockout embryo at E9.5. Cells were dissociated with papain, quenched with FBS. Filtered and resuspended in 30ul of DMEM +10%FBS. Cell suspension was put into 10x genomics V3 chemistry for single cell 3' sequencing.