Investigation of Interaction Partners of Desmin and Lamin B Proteins Using High Resolution Mass Spectroscopy

The aim of the project is to identify the proteins expected to be participated in desmin-lamin B interaction by high resolution mass spectrometry. Co-immunoprecipitation assay was performed using zebrafish skeletal muscle tissue followed by mass spectrometry to identify interacting proteins. In silico analysis was conducted to identify common proteins for desmin and lamin B and investigate the pathways that common proteins involved.