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Homo sapiens strain:U2OS.EGFP Targeted Locus (Loci)

NIAID Data Ecosystem2026-03-10 收录

CRISPR脱靶分析

基因编辑安全性

数据链接：

https://www.ncbi.nlm.nih.gov/sra/SRP033215 数据链接链接失效反馈

官方服务：

资源简介：

Human off-target analysis for CRISPR-Cas RNA-guided nucleases for gRNAS to VEGFA (Site 1 & 3) and EMX1 for gRNAs, trugRNAs, dual nickases, and tru dual nickases

应用场景：

创建时间：

2017-11-21

基因编辑安全性

人类胚胎基因编辑

We performed whole-genome sequencing (WGS) on human embryonic stem cells derived from human 8-cell embryos injected with or without FNLS-YE1, together with sgRNA that targeting PCSK9. Blood cell genom

NIAID Data Ecosystem90

Table_3_Gene Editing and Genotoxicity: Targeting the Off-Targets.XLSX

基因编辑安全性

脱靶效应检测

Gene editing technologies show great promise for application to human disease as a result of rapid developments in targeting tools notably based on ZFN, TALEN, and CRISPR-Cas systems. Precise modifica

NIAID Data Ecosystem80

Genotoxic effects of base and prime editing in human hematopoietic stem cells [WES opt]

基因编辑安全性

造血干细胞

Emerging base and prime editing may provide safer and more precise genetic engineering than nuclease-based approaches bypassing the dependence on DNA double strand breaks (DSBs). However, little is kn

NIAID Data Ecosystem60

No observable guide-RNA-independent off-target mutation induced by prime editor [RNA-Seq]

基因编辑安全性

全长转录组分析

Prime editor (PE) has been recently developed to induce efficient and precise on-target editing, whereas its guide RNA (gRNA)-independent off-target effects remain unknown. Here, we used whole-genome

NIAID Data Ecosystem90

Genome-wide detection of DNA double-stranded breaks induced by engineered nucleases

基因编辑安全性

核酸酶脱靶检测

We describe a robust linear amplification-mediated high-throughput genome-wide translocation sequencing (HTGTS) method that identifies endogenous or ectopic "prey" DNA double-stranded breaks (DSBs) ac

NIAID Data Ecosystem70

Homo sapiens strain:U2OS.EGFP Targeted Locus (Loci)

资源简介：

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