VCF for neutral data set in Harpagifer bispinis along the Magellan Province

Quaternary glacial cycles shaped the current distribution of polar and cold-temperate biotas. In the Magellan province of South America, ice covering during the last glacial maximum radically altered the landscape/seascape, speciation rate, and the distribution of species. Here we studied nototheniid fishes Harpagifer spp. along the Magellan province reported as two nominal species: H. bispinis in Patagonia and H. palliolatus, endemic to the Falkland/Malvinas Islands. Previous molecular analyses in Harpagifer showed that the genus may have recently colonized southern South America ~ 1 million years ago. The extensive use in systematics of molecular markers to determine evolutionary units has been improved due to the advances in NGS. Combining traditional DNA sequences and non-targeted GBS-SNPs we evaluated both, the presence of effective evolutionary units and contemporary patterns of genetic structure across the Magellan province. DNA sequences consistently showed an absence of phyloge...第四纪冰期循环塑造了极地与冷温生物群的现有分布格局。在南美洲麦哲伦省，末次冰盛期的冰川覆盖彻底改变了当地的陆海景观、物种形成速率与物种分布范围。本研究针对麦哲伦省境内的南极鱼科（Nototheniidae）叉棘杜父鱼属（Harpagifer）物种展开，该类群此前被记载为两个命名物种：分布于巴塔哥尼亚的双棘叉棘杜父鱼（Harpagifer bispinis），以及仅为福克兰/马尔维纳斯群岛特有的Harpagifer palliolatus。此前针对叉棘杜父鱼属的分子分析显示，该属可能在约100万年前才新近扩散至南美南部地区。得益于下一代测序（Next-Generation Sequencing, NGS）技术的发展，利用分子标记界定演化单元的分类学应用已得到大幅优化。本研究结合传统DNA序列数据与非靶向GBS-SNPs标记，对麦哲伦省境内的有效演化单元存在性以及当代遗传结构格局展开了评估。DNA序列分析一致显示，其系统地理...