Expression data from B6-sst1S and B6(WT) mouse bone-marrow derived macrophages

Using forward genetics in mice, we have identified a novel host resistance gene Ipr1, which mediates resistance to tuberculosis. We have shown that Ipr1-mediated pathway is regulated by IFNg in macrophages and plays an important role in resistance to several intracellular bacterial pathogens: Listeria, Francisella and Chlamydia. Functional studies of sst1 locus revealed that it plays a novel role in integrating signals generated by intracellular pathogens or viruses with mechanisms regulating activation, gene expression and cell death of host cell. Thus performing RNA-seq from sst1R and sst1S macrophages (BMDM) will help to uncover the global repertoire of events regulated by the sst1 locus and lead to the discovery of novel regulatory pathways necessary for host defense, thus providing a platform for developing novel therapeutics to improve host recovery after infections. We used microarrays to detail the global programme of gene expression mediated by the cytokine TNFα and identified upregulation of several important regulatory genes important for the susceptible phenotype. Bone marrow derived macrophages from B6-sst1S and B6(WT) treated with 10ng/ml TNFα for 18hrs were selected for RNA extraction and hybridization on Affymetrix microarrays.