SNP amplicons results of 50 hybrid Chinook-Coho salmon

These SNP panel results confirm the hybrid origin in 50 Chinook-Coho Salmon individuals. The SNP panel is composed of two amplicons and five diagnostic SNPs. DNA amplicons OkiOts_120255 and Oki_RAD41030 have SNP sites fixed for alternate base pairs in Chinook and Coho salmon (Beacham and Wallace 2019). The panel examined genotypes at one diagnostic position in OkiOts_120255 SNP: 113 (Reference=A, Variant=C) and four positions in Oki_RAD41030: 45 (TC), 51(CG), 195 (GA), and 198 (TG) called via Proton software Variant Caller®. The hybrid salmon were heterozygous for a Chinook and Coho haplotype at both SNP loci, confirming these as the parental species involved in the hybridization and consistent with all being F1 or higher order (F2 or back-cross) hybrid individuals. Beacham, T. D. & Wallace, C. G. (2019). Salmon species identification via direct DNA sequencing of single amplicons. Conservation Genetics Resources, 1-7. https://doi.org/10.1007/s12686-o19-01102-1.本单核苷酸多态性（Single Nucleotide Polymorphism, SNP）分型套件的检测结果证实，50尾大鳞大麻哈鱼（Chinook Salmon）与银大麻哈鱼（Coho Salmon）的杂交个体确为杂交起源。该SNP分型套件包含2个扩增子（amplicon）与5个诊断性SNP位点。扩增子OkiOts_120255与Oki_RAD41030的SNP位点在大鳞大麻哈鱼和银大麻哈鱼中分别固定了互斥的碱基对（Beacham & Wallace, 2019）。本套件针对OkiOts_120255中的1个诊断性SNP位点（113位，参考碱基为A，变异碱基为C）以及Oki_RAD41030中的4个位点（45位TC、51位CG、195位GA、198位TG）进行了基因型分型，上述位点均通过Proton软件变异呼叫器（Proton software Variant Caller®）完成碱基识别。杂交大麻哈鱼在两个SNP位点上均呈现大鳞大麻哈鱼与银大麻哈鱼单倍型的杂合状态，这证实二者为该杂交事件的亲本物种，且所有个体均符合子一代（F₁）或更高阶杂交个体（子二代F₂或回交个体）的基因型特征。 Beacham T D, Wallace C G. 2019. 基于单扩增子直接DNA测序的大麻哈鱼物种鉴定. 保护遗传学资源, 1-7. https://doi.org/10.1007/s12686-019-01102-1.