Supplementary Material for: Innate IgG Molecules and Innate B Cells Expressed by Immunoglobulin Constant Heavy G Chain (Fcγ) Genetic Marker Genes Are Involved in the ‘Allergic March' of IgE Sensitization in Children

<b><i>Background:</i></b> Interindividual variations of immunoglobulin constant heavy G chain <i>(IGHG)</i> genes on chromosome 14q32.3 are identified by alternative genetic markers (GM) of IgG3, IgG1 and IgG2, respectively. They express structurally and functionally innate IgG molecules and B cells, associated with allergic disease, replicated in several studies. <b><i>Materials and Methods:</i></b> 1-year-old and 10-year-old, IgE-sensitized and non-sensitized children from the German Multicenter Allergy Study birth cohort were assessed by new serological methods for the mendelian<i> IGHG</i> (Fcγ) (GM) genes, as innate IgG molecules and innate B cells. <b><i>Results:</i></b> Food allergy sensitization in thirty-five 1-year-old children (124 not sensitized) was associated with the <i>IGHG</i>*<i>bfn</i> haplotype and B*^bfn cells (OR 1.9, 95% CI 1.2-3.1; p = 0.010). Aeroallergen sensitization in ninety-nine 10-year-old children (95 not sensitized) was associated with the same genes (OR 1.4, 95% CI 1.02-1.9; p = 0.034). The IgE sensitization was most prominent in the restrictive homozygous <i>IGHG</i>*<i>bfn/</i>*<i>bfn </i>diplotype, 34% at age 1, increasing to 60% at age 10, rating the highest numbers of positive IgE tests, expressing increased levels of IgE and innate IgG2*n. <b><i>Conclusions:</i></b> The <i>IGHG</i>*<i>bfn </i>haplotype (B*^bfn cells) and increased innate IgG2*n levels are predictive factors for IgE sensitization in childhood. <i>IGHG</i> genes can be assessed for prognostic and preventive purposes in clinical care.