Targeting androgen receptor phase separation to overcome antiandrogen resistance mediated by "pharmacological condensates"

Analyze the transcriptomic changes of LNCaP upon DMSO, DHT, Enzalutamide(ENZ), ET516 treatment. The DHT treatment induced robust androgen receptor (AR) signaling up-regulation, wheras ENZ and ET516 can significantly restore DHT-induced AR signaling changes. LNCaP cells were treated with 10 μM ET516/ENZ/DMSO (DHT was added 2 hours after the compound) for 12 hours. Subsequently RNA were extracted and sequencing libraries were prepared for high thourghput sequencing.