Crystal structure of SCLam E144S mutant, a non-specific endo-beta-1,3(4)-glucanase from family GH16, co-crystallized with cellohexaose, presenting a 1,3-beta-D-cellobiosyl-glucose at active site

Crystal structure of SCLam E144S mutant, a non-specific endo-beta-1,3(4)-glucanase from family GH16, co-crystallized with cellohexaose, presenting a 1,3-beta-D-cellobiosyl-glucose at active site Descriptor: CALCIUM ION, GH16 family protein, beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-3)-alpha-D-glucopyranose Authors: Liberato, M.V, Squina, F. Deposit date: 2020-07-09 Release date: 2021-02-10 Last modified: 2024-10-23 Method: X-RAY DIFFRACTION (1.97 Å) Cite: Insights into the dual cleavage activity of the GH16 laminarinase enzyme class on beta-1,3 and beta-1,4 glycosidic bonds. J.Biol.Chem., 296, 2021

GH16家族非特异性内切β-1,3(4)-葡聚糖酶（endo-beta-1,3(4)-glucanase）SCLam E144S突变体的晶体结构，该蛋白与纤维六糖（cellohexaose）共结晶，活性位点结合有1,3-β-D-纤维二糖基-葡萄糖（1,3-beta-D-cellobiosyl-glucose）。描述项：钙离子（CALCIUM ION）、GH16家族蛋白（GH16 family protein）、β-D-吡喃葡萄糖-(1→4)-β-D-吡喃葡萄糖-(1→3)-α-D-吡喃葡萄糖（beta-D-glucopyranose-(1-4)-beta-D-glucopyranose-(1-3)-alpha-D-glucopyranose）。作者：Liberato, M.V、Squina, F.。沉积日期：2020-07-09；发布日期：2021-02-10；最后修改日期：2024-10-23。实验方法：X射线衍射（X-RAY DIFFRACTION），分辨率1.97埃（Å）。引用：《GH16昆布多糖酶类对β-1,3与β-1,4糖苷键的双重切割活性解析》，《生物化学杂志》（J.Biol.Chem.），296卷，2021年