Research data for article: Oxidation of lipid membrane cholesterol by cholesterol oxidase and its effects on raft model membrane structure

In this study, we investigate the effect of cholesterol oxidase on the model of lipid raft membrane model composed of DOPC:Chol:SM (1:1:1). The planar monolayers and liposomes were employed to follow membrane cholesterol oxidation to cholestenone catalyzed by ChOx and changes in the lipid membrane structure accompanying this reaction. Changes in the structure of liposomes in the presence of the enzyme were reflected in the changes in hydrodynamic diameter and fluorescence microscopy images. In contrast, changes in surface properties of planar membranes were evaluated by grazing incidence X-ray diffraction (GIXD) and Brewster angle microscopy. UV-Vis absorbance measurements confirmed the activity of the enzyme in the tested systems. A better understanding of the interactions between the enzyme and the cell membrane may help find alternative ways to decrease excessive cholesterol levels.