Quartet transcriptome reference materials for inter-laboratory proficiency test and data integration of transcriptomics profiling

As part of the Quartet Project for quality control and data integration of multiomics profiling, we established four RNA reference materials derived from immortalized B-lymphoblastoid cell lines from four members of a monozygotic twin family. Additionally, RNAseq datasets from the Quartet RNA reference materials were then collected, consisting of 252 RNAseq libraries from 21 batches generated in eight labs using two library construction protocols (poly(A) selection and RiboZero) and two sequencing platforms (Illumina NovaSeq and MGI DNBSEQ-T7).As part of the Quartet Project for quality control and data integration of multiomics profiling, we established four RNA reference materials derived from immortalized B-lymphoblastoid cell lines from four members of a monozygotic twin family. Additionally, miRNAseq datasets from the Quartet RNA reference materials were then collected, consisting of 72 miRNAseq libraries from six batches generated in four labs using five library kits (NEBNext, NEXTFLEX, TruSeq, Vazyme, and QIAseq) and two sequencing platforms (Illumina NovaSeq and HiSeq 2500).