Gene expression profile at single cell level of proximal tubular epithelial cell specific I-PpoI expressing mice kidney cortex

To investigate the alterations in cell populations in the kidney after proximal tubular epithelial cell DNA DSBs, we performed single-cell RNA sequencing (scRNA-seq) on the renal cortex of 16-week-old proximal tubular epithelial cell specific I-PpoI expressing mice. Kidneys were harvested, minced into 1 mm3 cubes, digested using digestion buffer and analyzed using scRNA-seq.