Quantification of metabolic enzymes in TIG-3 TSM and AIG cells

Normal human diploid fibroblast (TIG-3) cells were infected with retroviruses containing the early region of simian virus 40 (SV40) and encoding the catalytic component of human telomerase (hTERT) and human c-Myc. The resultant transformed cells (TSM cells) gained a moderate ability to undergo anchorage-independent growth. TSM clones grown in three dimensional (3-D) culture were picked up and expanded in 2-D culture to obtain AIG-1 clones. This selection process was repeated three times to yield AIG-3 cells.