Comparison of phage genome annotations performed using different methods.

Five phages, the canonical phage T1 and four previously annotated at the CPT, were assessed with RASTtk and features were compared. The number of gene features listed is strictly a count, not reflecting the possibility that completely different genes, or genes with alternate starts, were called. The hypothetical protein count is meant to be a proxy for functional annotation. Since RASTtk also uses the nonspecific name “phage protein”, this term was separately tallied. In the additional features row are listed genes and other aspects of phage genomes that are typically only identified by manual inspection, and which only our pipeline identifies here. tmp = tape measure protein, and terminators refer to rho-independent terminators predicted by TransTermHP. Compiled annotation data can be browsed by the public at https://cpt.tamu.edu/apollo/jbrowse/.
(XLSX)