Lentiviral transduction and FACS enrichment of hESCs.

(A) Lentiviral constructs used in this study. Structural details are provided in Supplemental Figures S3, S4, S5, S6, S7, S8, S9, S10 and Methods. (B,C) Merged brightfield phase contrast micrographs of mCherry-positive cells overlayed with fluorescence images illustrate the variegated expression in a colony before (b) and reduced variability after (c) FACS enrichment (see Materials and Methods). hESCs were maintained on unlabeled MEFs.