Validation assays. Summary of EMSA, promoter probe assays or RT-PCR.

(1)ND, not detected.
(2)Values are ratios of specific luminescences at 32 h of culture. Values are shown when t-test p-value <0.05 (4 biological replicates for each condition). NS, no significant differences. 1, 2, 3 and 4 as in Figure 2.
(3)Relative up or down expression of the gene in mutant strain.
(4)Luminescence measures too low (<1 arbitrary unit).
(5)Microarray results (when uncorrected p-value <0.05 for the contrast, if not, no comparison was done) showed the same sign of regulation.
(6)The probe does not correspond to the promoter region but to the upstream gene SCO2209 which contains the predicted ARG box.
(7)The predicted ARG box is located in the 25 nucleotides of the 3′end of SCO4159 (glnR).
(8)Predicted polycistronic transcript from SCO1488 (pyrR).
(9)Putative ARG boxes not found (Ri≥3, 800-nt region centered at the start codon).
(10)Predicted polycistronic transcript from SCO5583 (amtB).
(11)Predicted ARG box overlapping the start codon, Ri 7.7 bits.