Data and analyses from: Herbivory and time since flowering shape floral rewards and pollinator-pathogen interactions

Herbivory can induce chemical changes throughout plant tissues including flowers, which could affect pollinator-pathogen interactions. Pollen is highly defended compared to nectar, but no study has examined whether herbivory affects pollen chemistry. We assessed the effects of leaf herbivory on nectar and pollen alkaloids in Nicotiana tabacum, and how herbivory-induced changes in nectar and pollen affect pollinator-pathogen interactions. We damaged leaves of Nicotiana tabacum using the specialist herbivore Manduca sexta and compared nicotine and anabasine concentrations in nectar and pollen. We then pooled nectar and pollen by collection periods (within and after one month of flowering), fed them in separate experiments to bumble bees (Bombus impatiens) infected with the gut pathogen Crithidia bombi, and assessed infections after seven days. We did not detect alkaloids in nectar, and leaf damage did not alter the effect of nectar on Crithidia counts. In pollen, herbivory induced higher ...植食性取食可诱导植物包括花器官在内的全株组织发生化学变化，此类变化可能影响传粉者与病原菌间的互作关系。相较于花蜜，花粉具备更强的化学防御能力，但目前尚无研究探讨植食性取食是否会改变花粉的化学组成。本研究以普通烟草（Nicotiana tabacum）为对象，评估了叶片植食性取食对其花蜜与花粉中生物碱含量的影响，以及植食性诱导的花蜜、花粉成分变化如何作用于传粉者-病原菌互作。我们利用专食性植食昆虫烟草天蛾（Manduca sexta）对普通烟草叶片进行损伤处理，对比了其花蜜与花粉中的尼古丁与假木贼碱浓度。随后按采集时段（开花期内及开花后1个月）混合花蜜与花粉样本，在独立实验中将样本喂食给感染了肠道病原菌熊蜂短膜虫（Crithidia bombi）的熊蜂（Bombus impatiens），并于7天后评估其感染情况。本研究未在花蜜中检测到生物碱，且叶片损伤未改变花蜜对熊蜂短膜虫载量的影响。在花粉中，植食性取食诱导了更高的……