Fixed concentration evaluation of 11 candidate inhibitors in M.tuberculosis GS enzyme assays using the pre-incubation protocol.

The percentage enzyme inhibition obtained with the compounds (10μM final concentration) in 4 separate screens is shown, along with the average inhibition for each compound over the 4 screens and the corresponding standard deviation (SD). The colour coding of the highlighted table cells is clarified in the bottom legend. Compounds were defined as selective for the adenylylated or deadenylylated form of the enzyme based on at least a 30% difference in inhibition.