Gene expression profile at single cell level of the cerebral cortex at different sleep need

Gene expression profile varies along with sleep/wake behavior. We used single nucleus RNA sequencing (snRNA-seq) to analyze differentially expressed genes depending on sleep need. Overall design: The primary motor cortex of ad lib sleep slept or sleep deprived mice, and the same brain region locally administrated with AAV expressing wild-type or gein-of-function-mutated SIK3 were analyzed using snRNA-seq.