Gene expression analysis of rat macrophages

We used microarray to compare gene expression between bone marrow-derived (BMDM), monocyte-derived (MDM), alveolar (AM), peritoneal (PM) and embryonic stem cell (ESC) derived rat macrophages. The expression of liver macrophages (Kupffer cells) were inferred by analysis of whole livers from Csf1r deficient rats. The transcriptional response of BMDM to LPS was also examined.本研究采用微阵列（microarray）技术，比较了骨髓来源巨噬细胞（bone marrow-derived macrophage, BMDM）、单核细胞来源巨噬细胞（monocyte-derived macrophage, MDM）、肺泡巨噬细胞（alveolar macrophage, AM）、腹膜巨噬细胞（peritoneal macrophage, PM）以及胚胎干细胞（embryonic stem cell, ESC）来源的大鼠巨噬细胞的基因表达差异。本研究通过分析Csf1r缺陷大鼠的全肝组织，间接推断肝巨噬细胞——库普弗细胞（Kupffer cells）的基因表达水平。同时，本研究还检测了骨髓来源巨噬细胞（BMDM）对脂多糖（lipopolysaccharide, LPS）的转录应答反应。