RNA seq analysis of human Fetal and adult derived Enterospheres

enterospheres (FEnS) from six aborted fetal’ small intestines, ranging in gestational age from 11 to 22.5 weeks ), and from adult duodenum AEnS were generated based on previously developed protocols for human organoids cultures . We sought to compare the global gene expression profile of FEnS and AEnS by whole transcriptome shotgun sequencing (WTSS) analysis. We evaluated FEnS (N=6) and AEnS (N=3) samples at a low passage, ranging from P6 to P14. Examination of 2 groups of enterospheres (organoids) from human intestine from Fetal and adult developmental age