Transcriptomic profiling of tumor-infiltrating Treg cells in Foxp3-Cre-YFP melanoma-bearing mice

Tregs restrain anti-tumor immunity and facilitate the evasion of tumor cells from immune surveillance, thus has long been considered as a promising target for anti-tumor therapy. Recent studies found that excessive type 1 immune responses (mainly mediated by IFN-? and IL-12 et al.) in the tumor microenvironment drive Treg fragility that downregulates FOXP3 but upregulates IFN-?, hereby compromises its suppressive function.The expression level of FOXP3 in Treg cells regulates its immunosuppressive function. Therefore, we sorted FOXP3-high and FOXP3-low Treg cells and strived to find their differentially expressed genes and their functional regulatory mechanisms. Overall design: For bulk RNA-sequencing, FOXP3-high and FOXP3-low Treg cells in the tumor infiltrated lymphocytes (TILs) were sorted from Foxp3-Cre-YFP mice bearing B16-F0 melanoma. RNA was extracted and used for RNA-Sequencing analysis.