Owenia fusiformis µCT scan

µCT scan of a specimen of Owenia fusiformis stained with PTA for one week. Scan and reconstruction were obtained with the following settings: [System] Scanner=SkyScan1272 Instrument S/N=15H09112 Software Version=1.1.10 Source Type=HAMAMATSU_L11871_20 Camera Type=XIMEA xiRAY16 Camera Pixel Size (µm)=7.4 Camera X/Y Ratio=1.0058 [Acquisition] Number Of Files= 940 Number Of Rows= 1640 Number Of Columns= 2452 Partial Width=OFF Image crop origin X=0 Image crop origin Y=0 Camera binning=2x2 Image Rotation=-0.00100 Optical Axis (line)= 932 Camera to Source (mm)=274.64085 Object to Source (mm)=50.22367 Source Voltage (kV)= 45 Source Current (µA)= 165 Image Pixel Size (µm)=2.706481 Scaled Image Pixel Size (µm)=2.706481 Image Format=TIFF Depth (bits)=16 Reference Intensity=57000 Exposure (ms)=1166 Rotation Step (deg)=0.200 Use 360 Rotation=NO Scanning position=12.126 mm Frame Averaging=ON (3) Random Movement=OFF (15) Flat Field Correction=ON FF updating interval=94 Geometrical Correction=ON Filter=No Filter Gantry direction=CC Rotation Direction=CC Type of Detector Motion=STEP AND SHOOT Scanning Trajectory=ROUND Number Of Horizontal Offset Positions=1 Number of connected scans=5 Current scan number=1 Number of lines to be reconstructed=1290 Scan duration=1h:16m:33s Maximum vertical TS=5.0 [Reconstruction] Reconstruction Program=NRecon Program Version=Version: 1.7.1.0 Reconstruction engine=InstaRecon Engine version=Version: 2.0.3.7 Reconstruction from batch=Yes Postalignment=5.50 Connected Reconstruction (parts)=5 Sub-scan post alignment [0]=-0.500000 Sub-scan post alignment [1]=3.000000 Sub-scan post alignment [2]=5.500000 Sub-scan post alignment [3]=5.500000 Sub-scan post alignment [4]=9.000000 Sub-scan scan length [0]=1292 Sub-scan scan length [1]=1290 Sub-scan scan length [2]=1292 Sub-scan scan length [3]=1289 Sub-scan scan length [4]=1290 Used extra shift in X per scan(micron)= 0.000 9.266 21.355 35.921 42.377 Used extra shift in Y per scan(micron)= 0.000 -18.235 -28.164 -29.070 -33.034 Reconstruction servers= SKYSCAN1272 First Section=359 Last Section=5289 Reconstruction duration per slice (seconds)=0.298839 Total reconstruction time (3875 slices) in seconds=1158.000000 Section to Section Step=1 Sections Count=4931 Result File Type=TIF Result File Header Length (bytes)=12 Result Image Width (pixels)=2252 Result Image Height (pixels)=2220 Pixel Size (µm)=2.70648 Reconstruction Angular Range (deg)=188.00 Use 180+=OFF Angular Step (deg)=0.2000 Smoothing=3 Smoothing kernel=2 (Gaussian) Ring Artifact Correction=28 Draw Scales=OFF Object Bigger than FOV=OFF Reconstruction from ROI=ON ROI Top (pixels)=2305 ROI Bottom (pixels)=82 ROI Left (pixels)=117 ROI Right (pixels)=2369 ROI reference length=2452 Filter cutoff relative to Nyquist frequency=100 Filter type=0 Filter type description=Hamming (Alpha=0.54) Undersampling factor=1 Threshold for defect pixel mask (%)=0 Beam Hardening Correction (%)=64 CS Static Rotation (deg)=0.00 Minimum for CS to Image Conversion=0.038815 Maximum for CS to Image Conversion=0.506935 HU Calibration=OFF BMP LUT=0 Cone-beam Angle Horiz.(deg)=7.559776 Cone-beam Angle Vert.(deg)=5.060350 Automatic matching in Z=50 Automatic matching in X/Y=50 Automatic matching in rotation=0.000000 Automatic fusion=1 Data size has been reduced by conversion from 16-bit to 8-bit and decreasing the scale of the images.