Spectral algal fingerprinting and long sequencing in synthetic algal-microbial communities

Full-spectrum cytometry offers a novel technique in environmental research, enabling rapid, high-throughput analysis of single algal cells from different taxa by capturing entire emission spectra. In this work we demonstrate label-free identification and enumeration of phytoplankton, overcoming traditional methods limitations. We propose a strategy for monitoring synthetic algal communities based on integration of high-throughput spectral cytometry and long sequencing analysis.