GigaMUGA genotypes for 72 house mice collected in grain-growing regions of southeastern Australia

The management of invasive species has been greatly enhanced by population genetic analyses of multilocus single-nucleotide polymorphism (SNP) datasets that provide critical information regarding pest population structure, invasion pathways, and reproductive biology. For many applications, there is a need for protocols that offer rapid, robust, and efficient genotyping on the order of hundreds to thousands of SNPs, that can be tailored to specific study populations, and that are scalable for long-term monitoring schemes. Despite its status as a model laboratory species, there are few existing resources for studying wild populations of house mice (Mus musculus spp.) that strike this balance between data density and laboratory efficiency. Here we evaluate the utility of a custom-targeted capture genotyping-by-sequencing approach to support research on plaguing house mouse populations in Australia. This approach utilizes 3,651 hybridization capture probes targeting genome-wide SNPs identif..., Genomic DNA was extracted from mouse tissue samples (ear snips) and submitted to Neogen Inc. for genotyping using the GigaMUGA Illumina Infinium II platform. Data here are raw genotypes called using Illumina BeadStudio/GenomeStudio software, as provided by the genotyping service provider, and prior to any processing or filtering.,入侵物种管理领域借助多位点单核苷酸多态性（single-nucleotide polymorphism, SNP）数据集的群体遗传学分析，研究效能得到显著提升，此类数据集可提供有害生物群体结构、入侵路径及繁殖生物学相关的关键信息。针对诸多应用场景，亟需能够同时满足以下要求的实验方案：可对数百至数千个SNP位点实现快速、稳定且高效的基因分型，可针对特定研究群体进行定制化设计，且可适配长期监测计划的规模化需求。尽管小家鼠（Mus musculus spp.）已是成熟的模式实验物种，但目前能够兼顾数据密度与实验室操作效率的野生小家鼠群体研究资源仍较为匮乏。本研究评估了一款定制化靶向捕获测序分型技术的应用潜力，以支持针对澳大利亚境内成灾小家鼠群体的相关研究。该技术共设计3651个杂交捕获探针，靶向覆盖全基因组范围内已鉴定的SNP位点…… 研究人员从小鼠组织样本（耳组织剪样）中提取基因组DNA，并提交至Neogen公司，利用GigaMUGA Illumina Infinium II平台完成基因分型。本数据集所包含的原始基因型数据由基因分型服务提供商通过Illumina BeadStudio/GenomeStudio软件调用生成，且未经任何后续处理或过滤步骤。