Gene expression profiles of white bass (Morone chrysops) and hybrid striped bass (M. chrysops x M. saxatilis) gill tissue over time following Flavobacterium covae exposure

Columnaris disease is a prevalent disease in freshwater environments worldwide caused by the ubiquitous aquatic bacterium Flavobacterium species. Adhesion to the external mucosal surfaces of fishes is the initial stage of infection, and the gills specifically have been identified as both a primary target and release site for this pathogen. Previous research has indicated that a predominant US aquaculture product, the hybrid striped bass (Morone chrysops x M. saxatilis), is more susceptible to infection with Flavobacterium columnare (covae) than the maternal white bass (M. chrysops) parental species. Therefore, to further elucidate the differences between these fish we conducted a transcriptomic profiling study examining the differences of gene expression in gill mucosal tissue over time after exposure to F. covae isolate LSU-066-04. Combined with previous work, these data provide a greater understanding of host immune response to a common pathogen in moronids. Overall design: Gill tissues were sampled from 48 indiviudals at four timepoints prior to (t= 0 hr control) or following exposure to Flavobacterium covae (t= 1 hr, 4 hr, and 24 hr post treatment) (n=24 white bass, WB, and 24 hybrid striped bass, HB, total: 6 of each sampled at each of the four timepoints)

淡水环境中广泛存在的嗜水气单胞菌属（Flavobacterium species）导致的柱状病（Columnaris disease）是全球范围内普遍流行的一种疾病。感染初期，病原体通过粘附于鱼类外部粘膜表面开始，其中鳃部已被确认为该病原体的主要靶点和释放位点。先前的研究表明，一种主要的美国水产养殖产品——杂交条纹鲈（Morone chrysops x M. saxatilis）——相较于母本的白鲈（M. chrysops）父种，对柱状嗜水气单胞菌（covae）的感染更为易感。因此，为了进一步阐明这两种鱼类之间的差异，我们开展了一项转录组学分析研究，考察了鱼类鳃部粘膜组织在接触F. covae分离株LSU-066-04后不同时间点的基因表达差异。结合先前的研究成果，这些数据有助于深入理解莫鲈科鱼类对常见病原体的免疫反应。总体设计：在接触Flavobacterium covae（处理前t=0小时对照组、处理后t=1小时、4小时和24小时）的四个时间点，从48个个体中采集鳃组织样本（n=24白鲈，WB，和24杂交条纹鲈，HB，总计每个时间点各采集6个样本）。