Characterization of the DNA methylome in retinal pigment epithelium (RPE) of adult mice

We investigated the epigenetic plasticity of adult murine RPE using whole-genome bisulfite sequencing (WGBS) Overall design: To study DNA methylation, two WGBS libraries were prepared using DNA from two independent RPE samples containing 150,000–200,000 cells. The WGBS libraries were sequenced and bisulfite sequence reads were aligned using the Bismark software package. Global methylation level in adult mouse RPE was measured and percentages of methylation of CpG, CHG, and CHH contexts (where H is A, C or T) were identified.