Mapping Gata2 enhancer-dependent developmental trajectories by single-cell RNA-sequencing

We compared single-cell transcriptomes of fetal liver myeloid progenitor pools (CMP/GMP pool) from Gata2 -77 enhancer mutant embryos and wildtype littermates. Gene expression patterns in -77-deficient mice demonstrated loss of erythroid, megakaryocyte and granulocyte progenitors and an increase in monocyte Lineage-Sca1-cKit+CD34+ cells from E14.5 fetal livers of two wild type and two -77-deleted embryos were processed for single-cell RNA sequening using 10x Genomics Chromium platform.