Data from: Reward-induced phasic dopamine release in the monkey ventral striatum and putamen

In-vivo voltammetry has successfully been used to detect dopamine release in rodent brains, but its application to monkeys has been limited. We have previously detected dopamine release in the caudate of behaving Japanese monkeys using diamond microelectrodes (Yoshimi 2011); however it is not known whether the release pattern is the same in various areas of the forebrain. Recent studies have suggested variations in the dopaminergic projections to forebrain areas. In the present study, we attempted simultaneous recording at two locations in the striatum, using fast-scan cyclic voltammetry (FSCV) on carbon fibers, which has been widely used in rodents. Responses to unpredicted food and liquid rewards were detected repeatedly. The response to the liquid reward after conditioned stimuli was enhanced after switching the prediction cue. These characteristics were generally similar between the ventral striatum and the putamen. Overall, the technical application of FSCV recording in multiple locations was successful in behaving primates, and further voltammetric recordings in multiple locations will expand our knowledge of dopamine reward responses.活体伏安法（in-vivo voltammetry）已成功应用于啮齿动物大脑的多巴胺释放检测，但其在猴类中的应用仍受局限。我们此前曾借助金刚石微电极（diamond microelectrodes），在执行行为任务的日本猴尾状核（caudate）内检测到多巴胺释放（Yoshimi, 2011），但目前尚不明确前脑（forebrain）不同区域的多巴胺释放模式是否一致。近期研究表明，投射至前脑各区域的多巴胺能投射（dopaminergic projections）存在差异。本研究中，我们采用已广泛应用于啮齿动物研究的基于碳纤维的快速扫描循环伏安法（fast-scan cyclic voltammetry, FSCV），尝试对纹状体（striatum）的两个位点开展同步记录。实验中反复检测到动物对未预期食物与液体奖赏的响应信号；在切换预测线索（prediction cue）后，条件刺激（conditioned stimuli）所触发的液体奖赏响应显著增强。腹侧纹状体（ventral striatum）与壳核（putamen）的上述响应特征总体相似。综上，在执行行为任务的灵长类动物脑内开展多位点FSCV记录的技术方案已获成功，未来进一步推进多位点伏安法记录（voltammetric recordings）研究，将有助于深化我们对多巴胺奖赏响应（dopamine reward responses）机制的认知。