Digestion rates in larval Strongylocentrotus purpuratus during different larval feeding stages@en

Strongylocentrotus purpuratus (Stimpson 1857) originally collected in November 2020 from La Jolla, USA (Lat: 32.842674; Long: -117.257767) were held in flow-through tanks which were filled with water from Kiel Fjord adjusted to 31.5 psu at GEOMAR Helmholtz Centre for Ocean Research Kiel at 10 °C. For experimental characterization of protein digestion between January and June 2021 S. purpuratus larvae were generated by gently shaking adult males and females and held in climate chambers at 15°C and 31.5 psu in the Christian-Albrechts-Universität zu Kiel up to 14 days under different food conditions. Using RT-qPCR analysis, expression patterns of four protease genes including an enteropeptidase, aminopeptidase, carboxypeptidase and trypsin were analyzed against the housekeeping gene Elongation factor 1-alpha 1 (EF1a). Total protease activity of larval extracts were analyzed under different pHs, at different sampling points and with protease inhibitors including Soybean Trypsin Inhibitor and Aprotinin. Alterations of larval in vivo digestion rates were characterized under inhibitor influence. For both inhibitors the initial incubation before starting the reaction was 45 min. Data are supplement to: Hildebrand et al. (2023) Characterization of digestive proteases in the gut of a basal deuterostome accepted for publication in Journal of Experimental Biology.紫球海胆（Strongylocentrotus purpuratus (Stimpson 1857)）于2020年11月采集自美国拉霍亚（纬度：32.842674；经度：-117.257767），饲养于基尔亥姆霍兹海洋研究中心（GEOMAR Helmholtz Centre for Ocean Research Kiel）的流水养殖槽中，养殖用水为经调节至31.5实用盐度单位（psu）的基尔峡湾海水，水温维持10℃。2021年1月至6月期间，为开展蛋白质消化功能的实验表征研究，研究人员通过轻柔振摇成年雌雄个体获得紫球海胆（S. purpuratus）幼体，将其置于基尔大学（Christian-Albrechts-Universität zu Kiel）的气候培养箱中，以15℃、31.5 psu的条件培养至多14天，并设置不同饵料条件。本研究采用实时定量聚合酶链式反应（RT-qPCR），以持家基因延伸因子1-α1（Elongation factor 1-alpha 1, EF1a）作为内参，分析了4种蛋白酶基因的表达模式，包括肠肽酶、氨肽酶、羧肽酶及胰蛋白酶。同时检测了幼体提取物的总蛋白酶活性，测试了不同pH值环境与不同采样时间点下的活性变化，并使用大豆胰蛋白酶抑制剂（Soybean Trypsin Inhibitor）、抑肽酶（Aprotinin）等蛋白酶抑制剂开展干预实验。此外还表征了抑制剂存在条件下幼体体内消化速率的变化，两种抑制剂的反应前预孵育时间均为45分钟。本数据集为Hildebrand等人（2023年）的论文《原始后口动物肠道消化蛋白酶的表征》的补充数据，该论文已被《Journal of Experimental Biology》接收待发表。