hPIV3-C protein expression increases EGF signaling.

As described in Figure 2, HEK-293T, Hela, Vero, A549 or BEAS-2B cells were transfected with pFA2-Elk1, pGal4-UAS-Luc, pRL-CMV to measure the activation level of MAPK/ERK signaling pathway. Cells were co-transfected with plasmids encoding 3×FLAG-tagged MV-C, hPIV3-C or Nipah-C or the corresponding empty vector pCI-neo-3×FLAG. 12 h after transfection, cells were starved and stimulated 6 h later with 100 ng/ml of EGF. After 24 h, expression of luciferase was quantified. Results were normalized so that reporter activity in cells transfected with a control vector equals 1. Experiments were performed in triplicates and data represent means±SD.