Series 1C: Supplemental experiments on Tp-1335: Determination of dark acclimatization time of the diatom Thalassiosira pseudonana

<p>Experiments were conducted to investigate the combined effect of light and temperature changes on the growth rate (mu) of <em>Thalassiosira</em> <em>pseudonana</em> CCMP 1335. <em>T.</em> <em>pseudonana</em> was grown in artificial seawater (ASW) (Kester et al.1967), enriched as in f/2 (Guillard 1975). Each of five experiments per series was conducted at a different temperature (13.4; 18.5; 24.4; 26.5; 22.5 C). At each temperature, cultures were kept on a 12-hour light 12-hour dark cycle under eight light intensities ranging from 35 umol/m^2/S&nbsp;to 140 umol/m^2/s. Optical density measurements (OD680 and OD720), dark-adapted Instantaneous Chlorophyll Fluorescence (F0) and the quantum yield (QY=Fv/Fm, where Fv is the maximal variable fluorescence and Fm is the maximal fluorescence intensity) and cell concentrations were determined daily at the end of the dark period. Cell counts were conducted in a hemocytometer on a microscope.</p>