Kras activation in endometrial organoids drives cellular transformation and epithelial-mesenchymal transition

We found that in KrasG12D-expressing endometrial organoids, Pten knockdown did not confer tumorigenicity, but Cdkn2a knockdown or Trp53 deletion led to the development of carcinosarcoma (CS), a rare, aggressive tumor comprising both carcinoma and sarcoma.To better characterize the pathogenesis of CS from organoids, we conducted a transcriptome analysis. A total of 8 samples. For KrasG12D organoids with both Cdkn2a knockdown (#4) and Trp53 deletion (#5, #6), the pre-inoculated organoids and tumor-deribved organoids were analyzed. KrasG12D organoids with shLuc were also analyzed. Endometrial organoids without lentiviral infection served as reference.