Cellular responses to long-term phosphate starvation of fission yeast: Maf1 determines fate choice between quiescence and death associated with aberrant tRNA biogenesis (CHX experiments)

This RNA-Seq analysis compares gene expression of fission yeast cells (Schizosaccharomyces pombe) at various times after phosphate starvation (2, 4, and 8 HR) either with 100 µg/ml cycloheximide or without drug contrasting to the cells grown in phosphate replete conditions (0HR) in the absence of the drug. Overall design: 6 test samples (cycloheximide or no drug treated phosphate starved cells) were analyzed; 1 control sample (no drug, phosphate replete cells); 3 biological replicate for each sample were processed