Real-time RT-PCR data for nine genes selected for verification of microarray results

Because of the low number of biological replicates, no valid p-values could be estimated, so a gene with M>1 was considered to be differentially expressed in this analysis. All genes with M>1 showed the same direction of regulation (up or down-regulated by stress treatment) as on the microarray. The nine genes were selected based on their high M- and B-values in the microarray analysis. Relative expression levels (M-values) were estimated using β-actin as an internal control. Negative M-values indicate that the expression level was higher in control than in stress birds.