Effect of feeding on gene expression in HEK-293E cells either treated with Torin1 or not.

We examined how mTORC1 influences mRNA expression and alternative splicing during a growth response in human embryonic kidney (HEK) 293E cells, in which mTORC1 is not hyperactive. We released a starvation-induced growth arrest by refeeding, then analyzed mRNA levels and alternative splicing events one and four hours later under either control or Torin1 treatment conditions. To investigate the nutrient response on gene expression, we treated HEK-293E cells with either 1 or 4 hours of feeding, with or without Torin1 treatment.