File S1 - Identification of a Functionally Distinct Truncated BDNF mRNA Splice Variant and Protein in Trachemys scripta elegans

Figure S1. The cDNA sequence of tBDNF exons I, II, and III is shown as a partial sequence here for T. scripta elegans. The complete sequence for Chrysemys picta bellii can be obtained in the NCBI GenBank database (accession number AHGY01342430.1). The sequence for the common coding exon IV is shown complete. A partial sequence for tBDNF2a exon IV is shown to illustrate the deletion and is highlighted in yellow. The start and stop codons are shown in bold and underlined. Polyadenylation signals (PAS) are underlined. Sites for PCR and RLM-RACE primers are indicated: blue, inner and outer primers for exons 1–3 (Table S2); pink, 5′ RACE (Table S1); orange, 3′ RACE (Table S1). Table S1. Primers used for tBDNF 3′ and 5′ RACE analysis. Table S2. Primers used for RT-PCR of tBDNF transcripts. (DOC)