Chikungunya virus impacts the diversity of symbiotic bacteria in mosquito vector

Mosquitoes transmit numerous arboviruses including dengue and chikungunya virus (CHIKV). In recent years, mosquito species Aedes albopictus has expanded in the Indian Ocean region and was the principal vector of chikungunya outbreaks in La Reunion and neighbouring islands in 2005 and 2006. Vector-associated bacteria have recently been found to interact with transmitted pathogens. For instance, Wolbachia modulates the replication of viruses or parasites. However there has been no systematic evaluation of the diversity of the entire bacterial populations within mosquito individuals particularly in relation to virus invasion. Here, we investigated the effect of CHIKV infection on the whole bacterial community of Ae. albopictus. Taxonomic microarrays and quantitative PCR showed that members of Alpha-, and Gamma-proteobacteria phyla, as well as Bacteroidetes, responded to CHIKV infection. The abundance of bacteria from the Enterobacteriaceae family increased with CHIKV infection whereas the ...蚊虫可传播多种虫媒病毒（arbovirus），包括登革病毒与基孔肯雅病毒（chikungunya virus, CHIKV）。近年来，白纹伊蚊（Aedes albopictus）的分布范围在印度洋区域持续扩张，并在2005年与2006年成为留尼旺岛及周边岛屿基孔肯雅热暴发的主要传播媒介。近期研究发现，媒介相关细菌可与传播的病原体发生相互作用：例如沃尔巴克氏体（Wolbachia）能够调控病毒或寄生虫的复制过程。然而，目前尚未有针对蚊虫个体内完整细菌种群多样性的系统性评估，尤其是在病毒入侵相关的研究范畴中。本研究探究了基孔肯雅病毒感染对白纹伊蚊整体细菌群落的影响。分类学微阵列与定量聚合酶链式反应（quantitative PCR）结果显示，α-变形菌门（Alphaproteobacteria）、γ-变形菌门（Gammaproteobacteria）以及拟杆菌门（Bacteroidetes）的类群均会对基孔肯雅病毒感染产生应答。肠杆菌科（Enterobacteriaceae）细菌的丰度随基孔肯雅病毒感染程度升高，而……