Interleukin-4 Antagonizes Inflammation and Aging Caused Hematopoiesis Dysregulation

IL-4, not only drives Th2 cell differentiation and reprogram macrophages toward an anti-inflammatory state but also plays crucial roles in aging and immunotherapy. However, the role of IL-4-STAT6 signaling in hematopoietic stem and progenitor cells (HSPCs) are less studied. So we sequenced mRNA from IL-4-treated or STAT6-KO HSPCs, LT-HSCs and MPPs, to address the roles of IL-4-STAT6 signaling in hematopoiesis. Gene expression profiling analysis of RNA-seq data from HSCs/MPPs which isolated from different mice. For saline/IL-4c treatment, mice were injected with saline or 2.5µg/kg IL-4c(IL-4 and anti-IL-4 antibody complex) for 24 hours. For aged mice, 18-month-old mice were injected with saline or 2.5µg/kg IL-4c(IL-4 and anti-IL-4 antibody complex) for 24 hours.