Figure 5_Plaque Assay ARPE-19 NHDF

<b>Figure 5. Anti-gH antibodies significantly reduce plaque formation in infected cells. </b>The relative number of focus forming units/plaques (&gt;10,000 μm²) on Day 7 post infection in both ARPE-19 (a) and NHDF (b) are shown for each antibody at 10 and 0.5 ug/mL using M2E10 (α-IAV) and Cytogam® as negative and positive controls per plate. The % of plaques was determined using single colony verification with 10,000 μm² as the minimum colony area based on GFP expression in infected cells. All conditions were repeated using n=3 technical replicates and statistical significance was determined using Ordinary one-way ANOVA with multiple comparisons using Dunnett’s correction with single pooled variance comparing the relative number of plaques at 0.5 ug/mL of gH mAb to plaques in M2E10 treated wells. Statistical significance is denoted as *, p&lt;0.05; **, p&lt;0.01; ***, p&lt;0.001; ****, p&lt;0.0001. Error bars represent standard deviation from the mean.<b> </b>