Here, we engineered a modular, RNA-triggered fluorescence (RTF) reporter system de novo for spatiotemporal imaging of RNA from cellular to whole-plant in vivo . There are all all plasmid
Plasmid sequences in GenBank format. For visualization we recommend SnapGene software (from GSL Biotech; free SnapGene Viewer available at snapgene.com).
Bacterial plasmids are self-replicating, extrachromosomal elements associated with a variety of phenotypic traits including virulence, enhanced fitness, and resistance to antimicrobial agents. They pl