Monosaccharides quantified in porewater samples from coastal vegetated ecosystems

Porewater was taken from 30 to 50 cm depths in saltmarsh, seagrass and unvegetated areas around the German Bight in 2022 and 2023. Up to 9 points per ecosystem were sampled along a transect. Polysaccharides >5kDa were upconcentrated using AMICON-filtration devide and afterwards freeze dried. Dired samples were resuspended in MilliQ-water and acid hydrolysed (1 M HCl, 24 h, 100°C). Monosaccharides were analysed using anion exchange chromatography with pulsed amperometric detection (HPAEC-PAD, ThermoFisher Dionex ICS-5000+ system equipped with a CarboPac PA10 analytical column (2 x 250 mm) and a CarboPac PA10 guard column (2 x 50 mm)), according to Engel et al. (2011).2022至2023年，研究人员于德国湾周边的盐沼、海草床及无植被区域，采集了深度为30至50 cm的孔隙水（porewater）。沿样带对每个生态系统布设最多9个采样点进行采样。采用AMICON过滤装置浓缩分子量大于5 kDa的多糖，随后将样品进行冷冻干燥。将干燥后的样品重悬于密理博（MilliQ）超纯水中，并以1 M盐酸于100 ℃下水解24 h。单糖的分析参照Engel等（2011）的方法，采用配有脉冲安培检测器的阴离子交换色谱法（HPAEC-PAD，高效阴离子交换色谱-脉冲安培检测），使用赛默飞世尔（ThermoFisher）戴安ICS-5000+系统，配套CarboPac PA10分析柱（2 × 250 mm）与CarboPac PA10保护柱（2 × 50 mm）完成检测。