Xanthomonas biofilm biomass

Sheet 1: The rsmA mutant also has increased biofilm and cell adhesion on a glass surface when assessed by crystal violet staining. Sheet 2: A triple disruption of XC_1803/XC_2715/XC_2866 in an rsmA mutant background leads to reduction in biofilm to a level similar to that of the wild-type strain. In contrast mutation of XC_1803, XC_2715 and XC_2866 alone or in pairwise combination has little or no effect on biofilm formation in the rsmA mutant.工作表1：采用结晶紫染色法（crystal violet staining）检测结果显示，rsmA突变体在玻璃表面的生物膜（biofilm）形成量与细胞黏附水平均显著升高。 工作表2：在rsmA突变体的遗传背景下，对XC_1803、XC_2715及XC_2866进行三重基因敲除后，其生物膜形成能力可降至与野生型菌株（wild-type strain）相近的水平。与之相反，单独或成对敲除XC_1803、XC_2715及XC_2866，对rsmA突变体的生物膜形成几乎无显著影响。