Sphingobium indicum B90A nLC-MS/MS

The project aims to to understand the response of the lin genes in Sphingobium indicum B90A under the stress of HCH isomers and the metabolites formed during degradation of hexachlorocyclohexane (HCH). Entire cell proteome from Sphingobium indicum B90A was extracted in presence of four HCH isomers. Quantitative proteomics confirmed the constitutive expression of the linA, linB and linC genes of the HCH degradation pathway crucial for the initiation of HCH isomers degradation. LinM and LinN were upregulated in the presence of β- and δ-isomers suggested the important role of ABC transporter system in the depletion of β- and δ-HCH. Besides this HCH isomers induced oxidative stress caused systemic changes in strain B90A proteome.