Zooplankton community structure, diversity, and environmental context across the California Current Ecosystem based on dual-marker DNA metabarcoding (18S V4, COI) and paired Zooscan imaging during CCE-LTER Process Cruise P2107 (July–August 2021)

Zooplankton communities were sampled during the California Current Ecosystem Long-Term Ecological Research (CCE-LTER) Process Cruise P2107 (R/V Roger Revelle, July 21 – August 11 2021), spanning freshly upwelled to oligotrophic offshore waters. Oblique Bongo net tows (0.71 m diameter, 202 µm mesh) were collected to ~200 m depth, split quantitatively, size-fractionated (0.2-5 mm), and analyzed by paired morphological imaging (Zooscan) and dual-marker DNA metabarcoding targeting 18S V4 (Zhan et al. 2013) and COI (Leray et al. 2013). Environmental data (temperature, salinity, oxygen, fluorescence, nutrients,etc) were obtained from CTD casts and bottle samples. This dataset includes (1) raw read-count OTU tables for 18S and COI, (2) corresponding taxonomic assignments, and (3) environmental metadata matched by sample ID. These data provide high-resolution measurements of zooplankton community diversity, biomass proxies, and associated environmental gradients across the upwelling–offshore continuum of the California Current System.本数据集的浮游动物群落采样工作依托加州流生态系统长期生态研究（California Current Ecosystem Long-Term Ecological Research, CCE-LTER）P2107航次开展，搭载平台为“罗杰·雷维尔号”研究船，采样时段为2021年7月21日至8月11日，采样范围覆盖从新近上升流区至贫营养远洋水体的梯度区间。实验采用倾斜式邦戈网（网口直径0.71米，网孔孔径202微米）采集至约200米水深的拖网样品，经定量分装、粒径分级（0.2–5毫米）后，通过配对形态成像系统（Zooscan）与靶向18S V4区（Zhan等，2013）、COI区（Leray等，2013）的双标记DNA宏条形码（DNA metabarcoding）技术完成分析。环境数据（包括温度、盐度、溶解氧、荧光值、营养盐等）采自温盐深仪（CTD）剖面与采水器样品。本数据集包含三部分内容：(1) 18S与COI基因的原始读长计数操作分类单元（Operational Taxonomic Unit, OTU）表；(2) 对应的物种分类注释结果；(3) 以样品ID匹配的环境元数据。该数据集可为加州流系统上升流-远洋连续体范围内的浮游动物群落多样性、生物量替代指标及相关环境梯度提供高分辨率的观测数据。