Understanding gene expression profile of human brain primary endohtelial cell (HBMECs) treated with conditioned media (CM)

To investigate transcriptomic changes in human brain parymary endohtelial cells in response to conditioned media obtained from BNMN (unconditioned media), hiPSC-CN harboring swedish mutation (Swe22) and hiPSC-CN harboring isogneic control background (CVIA2). Overall, we are tyring to understand how EC transcriptome changes in reponse to Alzheimer's disease mimicking condition. Overall design: Comparative gene expression profiling analysis of RNA-seq data for primary HBMECs treated with conditioned media (BNMN, CVIA2 (SweWT/WT) and SWE22 (Swe+/+)) two batches of conditioned media (DEC and FEB) was treated.