Transcriptome analysis of reticulated platelets reveals a prothrombotic profile [mRNA-Seq]

To compare immature and mature platelet transcriptome Methods: reticulated or immature platelets(RPs) were defined as the platelets with the highest thiazole orange staining intensity (15% highest, RNAhigh) according to previous experiences and mature platelets were defined as the platelet with the 30% lowest thiazole orange staining intensity (RNAlow) and sorted (supplemental Figure S1D). We detected more than 1700 differentially expressed gene, incluning activation receptors RPs have a prothrombotic transcriptomic profile Platelets RNA was acquired frome healthy donors. And analyzed using using NextSeq-500