Data from: Multi-locus characterization of a woodrat (genus Neotoma) hybrid zone

In order to investigate hybridization between 2 species of woodrats, Neotoma floridana and N. micropus, 103 specimens were collected, in March of 1988, from a known area of sympatry, and compared to reference collections from areas of allopatry. Ten genetic markers, consisting of seven microsatellite loci, one mitochondrial gene (cytochrome-b [Cytb]), and two nuclear introns (intron 2 of the vertebrate alcohol dehydrogenase gene [Adh1-I2] and intron 7 of the beta-fibrinogen gene [Fgb-I7]) were used to develop a composite genotype for each individual, and for detection of hybridization. Six individuals were identified as pure parental N. micropus, 96 as hybrids, and one as pure parental N. floridana. Hybrids were formed primarily through matings between complex genotypes, resulting in a high prevalence of individuals classified as backcrosses. The ratio of hybrid classes, population substructure, and presence of significant linkage disequilibrium within the zone of contact could not reject either the hybrid-superiority or hybrid-equilibrium model as responsible for maintenance of this hybrid zone. The collection date of this dataset (1988) provided not only a point in time assessment of the hybrid zone, but also provided opportunities for future comparisons of temporal datasets with the purpose of examining hybrid zone characteristics over multiple generations.为探究两种林鼠——佛罗里达林鼠（*Neotoma floridana*）与小耳林鼠（*Neotoma micropus*）——的杂交现象，研究团队于1988年3月在已知的同域分布区域采集了103份标本，并与来自异域分布区域的参考标本集进行比对。本研究使用10个遗传标记构建每个个体的复合基因型以检测杂交，其中包含7个微卫星位点、1个线粒体基因（细胞色素b[Cytb]），以及2个核内含子：脊椎动物乙醇脱氢酶基因内含子2（Adh1-I2）与β-纤维蛋白原基因内含子7（Fgb-I7）。经鉴定，6份标本为纯合的小耳林鼠亲本个体，96份为杂交个体，仅1份为纯合的佛罗里达林鼠亲本个体。杂交个体主要由具有复杂基因型的亲本交配产生，回交个体在种群中占比极高。该杂交接触带内的杂交类群比例、种群亚结构以及显著的连锁不平衡现象，均无法否定杂交优势模型与杂交平衡模型对该杂交带维持机制的解释。本数据集的采集时间为1988年，不仅提供了该杂交带在单一时间点的现状评估，还为后续跨时间序列数据集的对比研究提供了契机，以探究多世代尺度下杂交带的特征动态。