Salmonella mediated actin condensation upon entry into HeLa and HepG2 cells

Cytoskeleton rearrangement by Salmonella Typhimurium wild type, lon mutant and lon complemented strains upon entry into the host cell, as investigated on HeLa and HepG2 cells under different stress conditions. Figures demonstrate changes in host cytoskeleton mediated by the trigger and zipper mechanisms in HeLa and HepG2 cells respectively. However, the deletion of lon gene did not cause significant changes in the host cytoskeleton as compared to the wild type and lon complemented strain, even though the strain was hyper-invasive due to lon deletion. Images demonstrate merged images taken by observing cells under a fluorescence microscope after staining with Phalloidin (actin filament stain) and DAPI (nuclear stain).

沙门氏菌鼠伤寒亚种野生型、lon突变型及其lon补体菌株在进入宿主细胞过程中引起的细胞骨架重组，研究在HeLa和HepG2细胞上不同应激条件下进行。图示分别展示了HeLa和HepG2细胞中由触发和接缝机制介导的宿主细胞骨架的变化。然而，与野生型和lon补体菌株相比，lon基因的缺失并未引起宿主细胞骨架的显著变化，尽管lon基因缺失导致菌株具有超侵袭性。图像展示了通过荧光显微镜观察染色后的细胞（使用Phalloidin（肌动蛋白纤维染色）和DAPI（核染色））所拍摄的合并图像。