Data: Impact of thermal stress during pupal development in a key pollinator

The increasing frequency and intensity of extreme thermal events like heatwaves, driven by climate change, threaten global biodiversity and entire ecosystems. Despite alarming projections of population declines for key pollinators like bumblebees, little is known about the direct impact of varying elevated temperatures during their pupal development. To gain a better understanding of the dose-response to thermal stress, we experimentally exposed Bombus terrestris L. (Hymenoptera: Apidae) pupae in vitro to varying thermal stress lengths (3-7 days) and intensities (36-38°C). This mechanistic approach allowed us to uniquely isolate the direct physiological effects of temperature on pupal development from complex colony dynamics and assess individual emergence success, developmental duration, body size, relative lipid content, and wing geometric morphometrics. At the critical threshold of 38°C, pupal emergence was drastically reduced (2-5 fold) and wing deformities increased (4-7 fold). Eve..., , # Impact of thermal stress during pupal development in a key pollinator

Dataset DOI: [10.5061/dryad.n02v6wx7z](10.5061/dryad.n02v6wx7z)

## Description of the data and file structure

This README provides information about the data files associated with the manuscript titled \"Impact of thermal stress during pupal development in a key pollinator\". These data files contain the datasets and RMD script on which our statistical analysis is based. 

### Files and variables

#### Data File: Data_ThermalStress_pupae.csv

This is the main data file, containing:

id = Bee ID\
colony =Colony name/ID\
collected = Date when 4th instar larva was collected\
treatment = Treatment group to which a larva was assigned\
temp = Temperature (in °C) the larva was exposed to\
dur = Duration (in days) for which the larva of exposed\
date_start = Date when larva pupated, and the treatment started\
date_hatching = Date when larva emerged as an adult\
date_death = Date when individual died\
emerged = Classifier ...,